Protein palmitoylation plays an important role in Trichomonas vaginalis adherence.
Nievas, Y. R., Vashisht, A. A., Corvi, M. M., Metz, S., Johnson, P. J., Wohlschlegel, J. A. and de Miguel, N.
From the double daggerLaboratorio de Parasitos Anaerobios, Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico Chascomus (IIB-INTECH), CONICET-UNSAM, Chascomus B7130IWA, Argentina.
section signDepartment of Biological Chemistry, University of California, Los Angeles, California, 90095-1489.
paragraph signLaboratorio de Bioquimica de Parasitos, Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico Chascomus (IIB-INTECH), CONICET-UNSAM, Chascomus B7130IWA, Argentina.
||Department of Microbiology, Immunology, and Molecular Genetics, University of California, Los Angeles, California, 90095-1489.
From the double daggerLaboratorio de Parasitos Anaerobios, Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico Chascomus (IIB-INTECH), CONICET-UNSAM, Chascomus B7130IWA, Argentina; ndemiguel@intech.gov.ar.
The flagellated protozoan parasite Trichomonas vaginalis is the etiologic agent of trichomoniasis, the most common non-viral sexually transmitted infection worldwide. As an obligate extracellular pathogen, adherence to epithelial cells is critical for parasite survival within the human host and a better understanding of this process is a prerequisite for the development of therapies to combat infection. In this sense, recent work has shown S-acylation as a key modification that regulates pathogenesis in different protozoan parasites. However, there are no reports indicating whether this post-translational modification is a mechanism operating in T. vaginalis In order to study the extent and function of S-acylation in T. vaginalis biology, we undertook a proteomic study to profile the full scope of S-acylated proteins in this parasite and reported the identification of 363 proteins involved in a variety of biological processes such as protein transport, pathogenesis related and signaling, among others. Importantly, treatment of parasites with the palmitoylation inhibitor 2-bromopalmitate causes a significant decrease in parasite: parasite aggregation as well as adherence to host cells suggesting that palmitoylation could be modifying proteins that are key regulators of Trichomonas vaginalis pathogenesis.
Molecular and Cellular Proteomics 17(11): 2229-2241 (2018)