Effects of heavy metals identified in Chascomus shallow lake on the endocrine-reproductive axis of pejerrey fish (Odontesthes bonariensis).
Garriz, A., Del Fresno, P. S., Carriquiriborde, P. and Miranda, L. A.
Laboratorio de Ictiofisiologia y Acuicultura, Instituto de Investigaciones Biotecnologicas Instituto Tecnologico de Chascomus "Dr. Raul Alfonsin", IIB-INTECH (CONICET- UNSAM), Calle Intendente Marino Km. 8.200 (B7130IWA), Chascomus, Buenos Aires, Argentina.
Centro de Investigaciones del Medio Ambiente (CIMA), Facultad de Ciencias Exactas, UNLP - CONICET, Calle 47 y 115, 1900 La Plata, Buenos Aires, Argentina.
Laboratorio de Ictiofisiologia y Acuicultura, Instituto de Investigaciones Biotecnologicas Instituto Tecnologico de Chascomus "Dr. Raul Alfonsin", IIB-INTECH (CONICET- UNSAM), Calle Intendente Marino Km. 8.200 (B7130IWA), Chascomus, Buenos Aires, Argentina. Electronic address: lmiranda@intech.gov.ar.
Some heavy metals related to human activities were measured in the water of Chascomus lake. The maximum concentrations were: 0.23 mug/L for Cd, 4.28 mug/L for Cr, 22.09 mug/L for Cu, 2.49 mug/L for Ni, 3.24 mug/L for Pb and 210.76 mug/L for Zn. The values of Cd, Cr, Cr, Pb and Zn were above the Argentine National Guidelines for the Protection of the Aquatic life. The analysis of gonadal condition of pejerrey fish (Odontesthes bonariensis) from this lake did not revealed any reproductive damages. However, exposures with environmental concentrations of Cd, Cr, Cu and Zn under laboratory conditions of pejerrey males (14 days), caused a significant increase of the expression of the three variants of gnrh in the brain (within Cd exposure) and a decrease in cyp19a1b mRNA (within Cu exposure). Furthermore, at pituitary level, a decrease in fshb transcript levels was observed in the fish exposed to Cd and Cr and i a decrease in the expression of both gonadotropin receptors at gonadal level in Zn exposure. Moreover, the gonads of the fish exposed to all the tested metals suffered structural damages showing shortness of the spermatic lobules, fibrosis, testis ova and the presence of piknotic cells. All these findings alert that heavy metals pollution affects the expression of key reproductive genes and gonadal structure of fish species that represent the predominant group of organisms and are considered sentinel species in the aquatic ecosystems.
General and Comparative Endocrinology : en prensa (2018)