Nesfatin-1 Regulates Feeding, Glucosensing and Lipid Metabolism in Rainbow Trout.
Blanco, A. M., Velasco, C., Bertucci, J. I., Soengas, J. L. and Unniappan, S.
Laboratory of Integrative Neuroendocrinology, Department of Veterinary Biomedical Sciences, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, SK, Canada.
Laboratorio de Fisioloxia Animal, Departamento de Bioloxia Funcional e Ciencias da Saude, Facultade de Bioloxia and Centro de Investigacion Marina, Universidade de Vigo, Vigo, Spain.
Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico Chascomus, Chascomus, Argentina.
Nesfatin-1 is an 82 amino acid peptide that has been involved in a wide variety of physiological functions in both mammals and fish. This study aimed to elucidate the role of nesfatin-1 on rainbow trout food intake, and its putative effects on glucose and fatty acid sensing systems. Intracerebroventricular administration of 25 ng/g nesfatin-1 resulted in a significant inhibition of appetite, likely mediated by the activation of central POMC and CART. Nesfatin-1 stimulated the glucosensing machinery (changes in sglt1, g6pase, gsase, and gnat3 mRNA expression) in the hindbrain and hypothalamus. Central fatty acid sensing mechanisms were unaltered by nesfatin-1, but this peptide altered the expression of mRNAs encoding factors regulating lipid metabolism (fat/cd36, acly, mcd, fas, lpl, pparalpha, and ppargamma), suggesting that nesfatin-1 promotes lipid accumulation in neurons. In the liver, intracerebroventricular nesfatin-1 treatment resulted in decreased capacity for glucose use and lipogenesis, and increased the potential of fatty acid oxidation. Altogether, the present results demonstrate that nesfatin-1 is involved in the homeostatic regulation of food intake and metabolism in fish.
Frontiers in Endocrinology 9: 484 (2018)