Altered levels of mitochondrial NFS1 affect cellular Fe and S contents in plants.
Armas, A. M., Balparda, M., Turowski, V. R., Busi, M. V., Pagani, M. A. and Gomez-Casati, D. F.
Centro de Estudios Fotosinteticos y Bioquimicos (CEFOBI-CONICET), Universidad Nacional de Rosario, 2000, Rosario, Argentina.
Instituto de Investigaciones Biotecnologicas, IIB-INTECH, CONICET-UNSAM, Chascomus, Argentina.
Centro de Estudios Fotosinteticos y Bioquimicos (CEFOBI-CONICET), Universidad Nacional de Rosario, 2000, Rosario, Argentina. gomezcasati@cefobi-conicet.gov.ar.
KEY MESSAGE: The ISC Fe-S cluster biosynthetic pathway would play a key role in the regulation of iron and sulfur homeostasis in plants. The Arabidopsis thaliana mitochondrial cysteine desulfurase AtNFS1 has an essential role in cellular ISC Fe-S cluster assembly, and this pathway is one of the main sinks for iron (Fe) and sulfur (S) in the plant. In different plant species it has been reported a close relationship between Fe and S metabolisms; however, the regulation of both nutrient homeostasis is not fully understood. In this study, we have characterized AtNFS1 overexpressing and knockdown mutant Arabidopsis plants. Plants showed alterations in the ISC Fe-S biosynthetic pathway genes and in the activity of Fe-S enzymes. Genes involved in Fe and S uptakes, assimilation, and regulation were up-regulated in overexpressing plants and down-regulated in knockdown plants. Furthermore, the plant nutritional status in different tissues was in accordance with those gene activities: overexpressing lines accumulated increased amounts of Fe and S and mutant plant had lower contents of S. In summary, our results suggest that the ISC Fe-S cluster biosynthetic pathway plays a crucial role in the homeostasis of Fe and S in plants, and that it may be important in their regulation.
Plant Cell Reports 38(8): 981-990 (2019)