Serological Approaches for Trypanosoma cruzi Strain Typing.
Balouz, V., Bracco, L., Ricci, A. D., Romer, G., Aguero, F. and Buscaglia, C. A.
Laboratory of Molecular Biology of Protozoa, Instituto de Investigaciones Biotecnologicas 'Dr Rodolfo Ugalde' (IIBio), Universidad Nacional de General San Martin (UNSAM)-CONICET, Buenos Aires, Argentina.
Trypanosomatics Laboratory, Instituto de Investigaciones Biotecnologicas 'Dr Rodolfo Ugalde' (IIBio), Universidad Nacional de General San Martin (UNSAM)-CONICET, Buenos Aires, Argentina.
Trypanosomatics Laboratory, Instituto de Investigaciones Biotecnologicas 'Dr Rodolfo Ugalde' (IIBio), Universidad Nacional de General San Martin (UNSAM)-CONICET, Buenos Aires, Argentina. Electronic address: fernan@iib.unsam.edu.ar.
Laboratory of Molecular Biology of Protozoa, Instituto de Investigaciones Biotecnologicas 'Dr Rodolfo Ugalde' (IIBio), Universidad Nacional de General San Martin (UNSAM)-CONICET, Buenos Aires, Argentina. Electronic address: cbuscaglia@iib.unsam.edu.ar.
Trypanosoma cruzi, the protozoan agent of Chagas' disease, displays a complex population structure made up of multiple strains showing a diverse ecoepidemiological distribution. Parasite genetic variability may be associated with disease outcome, hence stressing the need to develop methods for T. cruzi typing in vivo. Serological typing methods that exploit the presence of host antibodies raised against polymorphic parasite antigens emerge as an appealing approach to address this issue. These techniques are robust, simple, cost-effective, and are not curtailed by methodological/biological limitations intrinsic to available genotyping methods. Here, we critically assess the progress towards T. cruzi serotyping and discuss the opportunity provided by high-throughput immunomics to improve this field.
Trends in Parasitology 37(3): 214-225 (2021)