Inhibitors of the p38 cell signaling pathway as antiviral compounds against Junin virus.
Brunetti, J. E., Quintana, V. M., Scolaro, L. A. and Castilla, V.
Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), 1428, Buenos Aires, Argentina.
IQUIBICEN, Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET)-UBA, Ciudad Universitaria, 1428, Buenos Aires, Argentina.
Virus Immunology Group, Bernhard Nocht Institute for Tropical Medicine, Hamburg, Germany.
Instituto de Investigaciones Biotecnologicas, Universidad Nacional de San Martin, 1650, Buenos Aires, Argentina.
Laboratorio de Virologia, Departamento de Quimica Biologica, Facultad de Ciencias Exactas y Naturales, Universidad de Buenos Aires (UBA), 1428, Buenos Aires, Argentina. viviana@qb.fcen.uba.ar.
In the present study, we analyzed the modulation of p38 cell signaling by Junin virus (JUNV) and evaluated the antiviral activity of p38 inhibitors against JUNV. While JUNV induced a progressive activation of p38 throughout the infection in Vero cells, a partial downregulation of p38 phosphorylation was observed in HEK293 and HeLa cells. The compounds SB203580 and SB202190, which are selective inhibitors of p38, significantly reduced viral protein expression and viral yield in the cell lines examined, indicating that the p38 signaling pathway might be a promising antiviral target against JUNV infection.
Archives of Virology 167(3): 935-940 (2022)