Host diversification is concurrent with linear motif evolution in a Mastadenovirus hub protein.
Glavina, J., Rodriguez de la Vega, R. C., Risso, V. A., Leonetti, C. O., Chemes, L. B. and Sanchez, I. E.
CONICET - Universidad de Buenos Aires, Instituto de Quimica Biologica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Laboratorio de Fisiologia de Proteinas, Intendente Guiraldes 2160 - Ciudad Universitaria, CP1428 Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Quimica Biologica, Laboratorio de Fisiologia de Proteinas, Intendente Guiraldes 2160 - Ciudad Universitaria, CP1428 Buenos Aires, Argentina; Instituto de Investigaciones Biotecnologicas (IIBiO-CONICET), Universidad Nacional de San Martin, Av. 25 de Mayo y Francia, CP1650 Buenos Aires, Argentina. Electronic address: https://twitter.com/@JuliGlavi.
Ecologie Systematique Evolution, UMR8079, CNRS, AgroParisTech, Universite Paris-Saclay, 91400 Orsay, France. Electronic address: https://twitter.com/@LaPepena.
Departamento de Quimica Fisica, Facultad de Ciencias, Universidad de Granada, Granada, Spain; Unidad de Excelencia de Quimica Aplicada a Biomedicina y Medioambiente (UEQ), Universidad de Granada, Granada, Spain. Electronic address: https://twitter.com/@CesarLeonetti.
CONICET - Universidad de Buenos Aires, Instituto de Quimica Biologica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Laboratorio de Fisiologia de Proteinas, Intendente Guiraldes 2160 - Ciudad Universitaria, CP1428 Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Quimica Biologica, Laboratorio de Fisiologia de Proteinas, Intendente Guiraldes 2160 - Ciudad Universitaria, CP1428 Buenos Aires, Argentina.
Instituto de Investigaciones Biotecnologicas (IIBiO-CONICET), Universidad Nacional de San Martin, Av. 25 de Mayo y Francia, CP1650 Buenos Aires, Argentina. Electronic address: lchemes@iib.unsam.edu.ar.
CONICET - Universidad de Buenos Aires, Instituto de Quimica Biologica de la Facultad de Ciencias Exactas y Naturales (IQUIBICEN), Laboratorio de Fisiologia de Proteinas, Intendente Guiraldes 2160 - Ciudad Universitaria, CP1428 Buenos Aires, Argentina; Universidad de Buenos Aires, Facultad de Ciencias Exactas y Naturales, Departamento de Quimica Biologica, Laboratorio de Fisiologia de Proteinas, Intendente Guiraldes 2160 - Ciudad Universitaria, CP1428 Buenos Aires, Argentina. Electronic address: isanchez@qb.fcen.uba.ar.
Over one hundred Mastadenovirus types infect seven orders of mammals. Virus-host coevolution may involve cospeciation, duplication, host switch and partial extinction events. We reconstruct Mastadenovirus diversification, finding that while cospeciation is dominant, the other three events are also common in Mastadenovirus evolution. Linear motifs are fast-evolving protein functional elements and key mediators of virus-host interactions, thus likely to partake in adaptive viral evolution. We study the evolution of eleven linear motifs in the Mastadenovirus E1A protein, a hub of virus-host protein-protein interactions, in the context of host diversification. The reconstruction of linear motif gain and loss events shows fast linear motif turnover, corresponding a virus-host protein-protein interaction turnover orders of magnitude faster than in model host proteomes. Evolution of E1A linear motifs is coupled, indicating functional coordination at the protein scale, yet presents motif-specific patterns suggestive of convergent evolution. We report a pervasive association between Mastadenovirus host diversification events and the evolution of E1A linear motifs. Eight of 17 host switches associate with the gain of one linear motif and the loss of four different linear motifs, while five of nine partial extinctions associate with the loss of one linear motif. The specific changes in E1A linear motifs during a host switch or a partial extinction suggest that changes in the host molecular environment lead to modulation of the interactions with the retinoblastoma protein and host transcriptional regulators. Altogether, changes in the linear motif repertoire of a viral hub protein are associated with adaptive evolution events during Mastadenovirus evolution.
Journal of Molecular Biology 434(10): 167563 (2022)