Pluripotent Nontumorigenic Adipose Tissue-Derived Muse Cells have Immunomodulatory Capacity Mediated by Transforming Growth Factor-beta1.
Gimeno, M. L., Fuertes, F., Barcala Tabarrozzi, A. E., Attorressi, A. I., Cucchiani, R., Corrales, L., Oliveira, T. C., Sogayar, M. C., Labriola, L., Dewey, R. A. and Perone, M. J.
Instituto de Investigacion en Biomedicina de Buenos Aires, National Scientific and Technical Research Council (CONICET), Partner Institute of the Max Planck Society, Buenos Aires, Argentina.
Servicio de Cirugia Plastica, Hospital Austral, Derqui, Argentina.
Biochemistry Department, Chemistry Institute, University of Sao Paulo, Sao Paulo, Brasil.
Cell and Molecular Therapy Center (Nucleo de Terapia Celular e Molecular/NETCEM), School of Medicine, University of Sao Paulo, Sao Paulo, Brasil.
Laboratorio de Terapia Genica y Celulas Madre, Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico de Chascomus (IIB-INTECH), National Scientific and Technical Research Council, National University of General San Martin, Chascomus, Argentina.
Adult mesenchymal stromal cell-based interventions have shown promising results in a broad range of diseases. However, their use has faced limited effectiveness owing to the low survival rates and susceptibility to environmental stress on transplantation. We describe the cellular and molecular characteristics of multilineage-differentiating stress-enduring (Muse) cells derived from adipose tissue (AT), a subpopulation of pluripotent stem cells isolated from human lipoaspirates. Muse-AT cells were efficiently obtained using a simple, fast, and affordable procedure, avoiding cell sorting and genetic manipulation methods. Muse-AT cells isolated under severe cellular stress, expressed pluripotency stem cell markers and spontaneously differentiated into the three germ lineages. Muse-AT cells grown as spheroids have a limited proliferation rate, a diameter of approximately 15 microm, and ultrastructural organization similar to that of embryonic stem cells. Muse-AT cells evidenced high stage-specific embryonic antigen-3 (SSEA-3) expression ( approximately 60% of cells) after 7-10 days growing in suspension and did not form teratomas when injected into immunodeficient mice. SSEA-3+ -Muse-AT cells expressed CD105, CD29, CD73, human leukocyte antigen (HLA) class I, CD44, and CD90 and low levels of HLA class II, CD45, and CD34. Using lipopolysaccharide-stimulated macrophages and antigen-challenged T-cell assays, we have shown that Muse-AT cells have anti-inflammatory activities downregulating the secretion of proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Muse-AT cells spontaneously gained transforming growth factor-beta1 expression that, in a phosphorylated SMAD2-dependent manner, might prove pivotal in their observed immunoregulatory activity through decreased expression of T-box transcription factor in T cells. Collectively, the present study has demonstrated the feasibility and efficiency of obtaining Muse-AT cells that can potentially be harnessed as immunoregulators to treat immune-related disorders. Stem Cells Translational Medicine 2017;6:161-173.
Stem Cells Transl Med 6(1): 161-173 (2017)