A comprehensive analysis of direct and photosensitized attenuation of Toxoplasma gondii tachyzoites.
Yanuk, J. G., Alomar, M. L., Gonzalez, M. M., Alonso, A. M., Angel, S. O., Coceres, V. M. and Cabrerizo, F. M.
Laboratorio de Fotoquimica y Fotobiologia Molecular, Instituto de Investigaciones Biotecnologicas - Instituto Tecnologico de Chascomus (IIB-INTECH), Universidad Nacional de San Martin (UNSAM) - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Av. Intendente Marino Km. 8.2, C.C 164, B7130IIWA Chascomus, Prov. Buenos Aires, Argentina.
Laboratorio de Parasitologia Molecular, Instituto de Investigaciones Biotecnologicas - Instituto Tecnologico de Chascomus (IIB-INTECH), Universidad Nacional de San Martin (UNSAM) - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Av. Intendente Marino Km. 8.2, C.C 164, B7130IIWA Chascomus, Prov. Buenos Aires, Argentina.
Laboratorio de Fotoquimica y Fotobiologia Molecular, Instituto de Investigaciones Biotecnologicas - Instituto Tecnologico de Chascomus (IIB-INTECH), Universidad Nacional de San Martin (UNSAM) - Consejo Nacional de Investigaciones Cientificas y Tecnicas (CONICET), Av. Intendente Marino Km. 8.2, C.C 164, B7130IIWA Chascomus, Prov. Buenos Aires, Argentina. Electronic address: fcabrerizo@intech.gov.ar.
In the present work, we have evaluated the effect of three different types of radiation: UVC (254+/-5nm), UVA (365+/-20nm) and visible (420+/-20nm) on different morphological and biological functions of Toxoplasma gondii tachyzoites. Briefly, UVC and UVA showed an inhibitory effect on parasite invasion in a dose-dependent manner. UVC showed the strongest effect inducing both structural damage (antigens) and functional inhibition (i.e., invasion and replication). On its own, visible light induces a quite distinctive and selective pattern of parasite-attenuation. This type of incident radiation inhibits the replication of the parasite affecting neither the capability of invasion/attachment nor the native structure of proteins (antigens) on parasites. Such effects are a consequence of photosensitized processes where phenol red might act as the active photosensitizer. The potential uses of the methodologies investigated herein are discussed.
Journal of Photochemistry and Photobiology 177: 8-17 (2017)