Phenotypic and Genotypic Characterization of Mutant Plants in Polyamine Metabolism Genes During Pathogenic Interactions.
Rossi, F. R., Romero, F. M., Ruiz, O. A., Marina, M. and Garriz, A.
Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico Chascomus, Universidad Nacional de General San Martin-Consejo Nacional de Investigaciones Cientificas y Tecnicas (IIB-INTECH/UNSAM-CONICET), Chascomus, Argentina. francorossi@intech.gov.ar.
Instituto de Investigaciones Biotecnologicas-Instituto Tecnologico Chascomus, Universidad Nacional de General San Martin-Consejo Nacional de Investigaciones Cientificas y Tecnicas (IIB-INTECH/UNSAM-CONICET), Chascomus, Argentina.
Plants respond to pathogen attack by modifying defense gene expression and inducing the production of myriad proteins and metabolites. Among these responses, polyamine (PA) levels suffer remarkable modifications. Evidences demonstrate that plants make use of the polyamine biosynthetic pathway and the oxidative catabolism of these compounds in order to mount adequate defenses against pathogens. In Arabidopsis thaliana, putrescine is synthesized exclusively through the arginine decarboxylase (ADC) pathway, this enzyme exists as two isoforms named ADC1 and ADC2. Even though both isoforms participate in the response to pathogen attack, the mechanisms modulating ADC activity are not completely understood. Therefore, studies to clarify their roles are necessary. In this chapter, we describe the methods that can be applied for the study of plant-pathogen interactions using Arabidopsis adc mutant plants.
Methods in Molecular Biology 1694: 405-416 (2018)