Sede San Martín

Director:

Dr. Javier G. De Gaudenzi

Investigador Adjunto CONICET
Profesor Adjunto UNSAM
[email protected]

Miembros actuales

Dra. Vanina A. Campo (Inv. Adjunta CONICET)
Lic. Karina Sabalette (Becaria doctoral CONICET)
Est. María Eugenia Intrieri (Estudiante UNSAM)
Belén Pachano (Estudiante UNSAM)

Regulones postranscripcionales: estudio de la expresión coordinada de genes.

Interacción entre elementos en cis y proteínas con dominios RRM.

Maduración de unidades precursoras de ARN mediante eventos regulados de trans-splicing.

Regulones de ARN controlan la expresión genética en tripanosomas
(De Gaudenzi et al, PeerJ 2013; http://peerj.com/articles/118).

1. RNA-binding proteins and mRNA turnover in trypanosomes.
   D’Orso I, De Gaudenzi JG, Frasch AC.
   TRENDS in Parasitology, 19(4):151-155 (2003)
2. RRM-type RNA-binding proteins in Trypanosoma cruzi form a family involved in the interaction with specific transcripts in vivo
   De Gaudenzi JG, D’Orso I, Frasch AC
   The Journal of Biological Chemistry, 278(21):18884-18894 (2003)
3. The Genome of the Kinetoplastid Parasite, Leishmania major
   Ivens AC, Peacock CS, Worthey EA, Murphy L, Aggarwal G, Berriman M, Sisk E, Rajandream MA, Adlem E, Aert R, Anupama A, Apostolou Z, Attipoe P, Bason N, Bauser C, Beck A, Beverley SM, Bianchettin G, Borzym K, Bothe G, Bruschi CV, Collins M, Cadag E, Ciarloni L, Clayton C, Coulson RM, Cronin A, Cruz AK, Davies RM, De Gaudenzi JG, Dobson DE, Duesterhoeft A, Fazelina G, Fosker N, Frasch AC, Fraser A, Fuchs M, Gabel C, Goble A, Goffeau A, Harris D, Hertz-Fowler C, Hilbert H, Horn D, Huang Y, Klages S, Knights A, Kube M, Larke N, Litvin L, Lord A, Louie T, Marra M, Masuy D, Matthews K, Michaeli S, Mottram JC, Muller-Auer S, Munden H, Nelson S, Norbertczak H, Oliver K, O'neil S, Pentony M, Pohl TM, Price C, Purnelle B, Quail MA, Rabbinowitsch E, Reinhardt R, Rieger M, Rinta J, Robben J, Robertson L, Ruiz JC, Rutter S, Saunders D, Schafer M, Schein J, Schwartz DC, Seeger K, Seyler A, Sharp S, Shin H, Sivam D, Squares R, Squares S, Tosato V, Vogt C, Volckaert G, Wambutt R, Warren T, Wedler H, Woodward J, Zhou S, Zimmermann W, Smith DF, Blackwell JM, Stuart KD, Barrell B, Myler P
   Science, 309: 436-442 (2005)
4. RNA-binding domain proteins in Kinetoplastids: a comparative analysis
   De Gaudenzi JG, Frasch AC, Clayton CE
   Eukaryotic Cell, 4(12):2106-2114 (2005)
5. RRM proteins in trypanosomes: possible targets for new drugs?
   De Gaudenzi JG, Frasch AC, Clayton CE
   Microbe, 1(2):82 (2006)
6. mRNA maturation by two step trans-splicing/polyadenylation processing in trypanosomes
   Jäger AV*, De Gaudenzi JG*, Cassola A, D’Orso I, Frasch AC
   Proc Natl Acad Sci U S A, 104(7):2035-2042 (2007)
   *Ambos primeros autores
7. Recruitment of mRNAs to cytoplasmic ribonucleoprotein granules in trypanosomes
   Cassola A, De Gaudenzi JG, Frasch AC
   Molecular Microbiology, 65(3):655-670 (2007)
8. Functionally related transcripts have common RNA motifs for specific RNA-binding proteins in trypanosomes
   Noé G, De Gaudenzi JG, Frasch AC
   BMC Molecular Biology 9:107 (2008)
9. Hyperosmotic stress induces aquaporin-dependent cell shrinkage, polyphosphate synthesis, amino acid acummulation and global gene expression changes in Trypanosoma cruzi
   Li ZH, Alvarez V, De Gaudenzi JG, Sant' Anna C, Frasch AC, Cazzulo JJ, Docampo R
   The Journal of Biological Chemistry, 286(51):43959–43971 (2011)
10. Gene expression regulation in trypanosomatids
    De Gaudenzi JG#, Noé G, Campo VA, Frasch AC, Cassola A
    Essays in Biochemistry, 51, 31–46 (2011)
    #Autor para correspondencia
11. A 43-nt U-rich element in 3´UTR of large number of Trypanosoma cruzi transcripts is important for mRNA abundance in intracellular amastigotes
    Li ZH, De Gaudenzi JG, Alvarez V, Huang H, Mendiondo N, Kissinger, Frasch AC, Docampo R.
    The Journal of Biological Chemistry, 287(23): 19058–19069 (2012)
12. Genome-wide analysis of 3'-UTRs supports the existence of post-transcriptional regulons controlling gene expression in trypanosomes
    De Gaudenzi JG*, Carmona S, Agüero F, Frasch AC
    PeerJ, 1e118; doi: 10.7717/peerj.118 (2013)
    *Autor para correspondencia
13. Identification of novel cyclic nucleotide binding proteins in Trypanosoma cruzi Jäger AV*, De Gaudenzi JG*, Mild JG, Mc Cormack B, Pantano S, Altschuler DL, Edreira ME Mol Biochem Parasitol, 198: 104-112 (2015)
    *Ambos primeros autores

• Alberto C. Frasch, IIB-INTECH
• Roberto Docampo, University of Georgia, Athens, USA
• Martín Edreira, Facultad de Ciencias Exactas, UBA
• Beatriz Garat, Facultad de Ciencias, Montevideo, Uruguay

• 2010-2012 PICT, ANPCyT
• 2011-2013 PIP IU, CONICET
• 2013-2015 PICT, ANPCyT
• 2013-2014 Fundación Bunge y Born
• 2014  Proyectos Puente 2013, UNSAM
• 2015-2016 Fundación Florencio Fiorini