Effects of In Vitro Interactions of Oviduct Epithelial Cells with Frozen-Thawed Stallion Spermatozoa on Their Motility, Viability and Capacitation Status.
Gimeno, B. F., Bariani, M. V., Laiz-Quiroga, L., Martinez-Leon, E., Von-Meyeren, M., Rey, O., Mutto, A. A. and Osycka-Salut, C. E.
Laboratorio de Biotecnologias Reproductivas y Mejoramiento Genetico Animal, Instituto de Investigaciones Biotecnologicas, Universidad Nacional de San Martin (UNSAM), Campus Miguelete, Avenida 25 de Mayo y Francia, San Martin, Buenos Aires, CP 1650, Argentina.
Signaling and Cancer Laboratory, Consejo Nacional de Investigaciones Cientificas y Tecnicas, Instituto de Inmunologia, Genetica y Metabolismo, Facultad de Farmacia y Bioquimica, Hospital de Clinicas "Jose de San Martin", Universidad de Buenos Aires, Ciudad Autonoma de Buenos Aires (CABA
Cryopreservation by negatively affecting sperm quality decreases the efficiency of assisted reproduction techniques (ARTs). Thus, we first evaluated sperm motility at different conditions for the manipulation of equine cryopreserved spermatozoa. Higher motility was observed when spermatozoa were incubated for 30 min at 30 x 10(6)/mL compared to lower concentrations (p < 0.05) and when a short centrifugation at 200x g was performed (p < 0.05). Moreover, because sperm suitable for oocyte fertilization is released from oviduct epithelial cells (OECs), in response to the capacitation process, we established an in vitro OEC culture model to select a sperm population with potential fertilizing capacity in this species. We demonstrated E-cadherin and cytokeratin expression in cultures of OECs obtained. When sperm-OEC cocultures were performed, the attached spermatozoa were motile and presented an intact acrosome, suggesting a selection by the oviductal model. When co-cultures were incubated in capacitating conditions a greater number of alive (p < 0.05), capacitated (p < 0.05), with progressive motility (p < 0.05) and with the intact acrosome sperm population was observed (p < 0.05) suggesting that the sperm population released from OECs in vitro presents potential fertilizing capacity. Improvements in handling and selection of cryopreserved sperm would improve efficiencies in ARTs allowing the use of a population of higher-quality sperm.
Animals (Basel) 11(1): en prensa (2021)