Pluripotent Core in Bovine Embryos: A Review.
Aguila, L., Osycka-Salut, C., Treulen, F. and Felmer, R.
Centre de Recherche en Reproduction et Fertilite (CRRF), Universite de Montreal, Saint-Hyacinthe, QC J2S 2M2, Canada.
Laboratory of Reproduction, Centre of Reproductive Biotechnology (CEBIOR-BIOREN), Universidad de La Frontera, Temuco 4811322, Chile.
Laboratorio de Biotecnologias Reproductivas y Mejoramiento Genetico Animal, Instituto de Investigaciones Biotecnologicas, Universidad Nacional de San Martin (UNSAM), Buenos Aires CP 1650, Argentina.
Escuela de Tecnologia Medica, Facultad de Medicina y Ciencias de la Salud, Universidad Mayor, Temuco 4801043, Chile.
Department of Agricultural Sciences and Natural Resources, Faculty of Agriculture and Forestry Sciences, Universidad de La Frontera, Temuco 4811322, Chile.
Early development in mammals is characterized by the ability of each cell to produce a complete organism plus the extraembryonic, or placental, cells, defined as pluripotency. During subsequent development, pluripotency is lost, and cells begin to differentiate to a particular cell fate. This review summarizes the current knowledge of pluripotency features of bovine embryos cultured in vitro, focusing on the core of pluripotency genes (OCT4, NANOG, SOX2, and CDX2), and main chemical strategies for controlling pluripotent networks during early development. Finally, we discuss the applicability of manipulating pluripotency during the morula to blastocyst transition in cattle species.
Animals (Basel) 12(8): en prensa (2022)