Development of a novel glycoprotein-based immunochromatographic test for the rapid serodiagnosis of bovine brucellosis.
Novak, A., Melli, L. J., Rey Serantes, D. A., Caillava, A. J., Comerci, D. J., Ugalde, J. E. and Ciocchini, A. E.
Instituto de Investigaciones Biotecnologicas "Dr. Rodolfo A. Ugalde", Universidad Nacional de San Martin (IIB-UNSAM, IIBIO-CONICET), Buenos Aires, Argentina.
Comision Nacional de Energia Atomica, Grupo Pecuario, Centro Atomico Ezeiza, Buenos Aires, Argentina.
AIMS: Bovine brucellosis is a worldwide zoonotic disease that causes important economic losses and public health concerns. Because control of the disease depends on vaccination, serodiagnosis and isolation of the infected animals, affordable, rapid and accurate point of care (POC) tests are needed. METHODS AND RESULTS: We developed and evaluated a novel glycoprotein-based immunochromatographic test for the detection of IgG antibodies against the O-polysaccharide of Brucella in bovine serum samples. Brucella GlycoStrip combines the power of immunochromatographic and bacterial glycoengineering technologies for the diagnosis of bovine brucellosis. The analysis of positive and negative reference samples indicated that the test has a diagnostic sensitivity and specificity of 96.9% (95% CI: 92.7%-100.0%) and 100%, respectively. CONCLUSIONS: Due to the recombinant glycoprotein-based antigen OAg-AcrA, which consists of the O-side chain of Brucella smooth lipopolysaccharide (sLPS) covalently linked to the carrier protein AcrA, the test is highly accurate, allows the differentiation of infected animals from those vaccinated with a rough strain or with a single dose of a smooth strain and fulfil the minimum diagnostic requirements established by the national and international regulations. SIGNIFICANCE AND IMPACT OF STUDY: This strip test could provide a rapid (10 min) and accurate diagnosis of bovine brucellosis in the field contributing to the control of the disease.
Journal of Applied Microbiology 132(6): 4277-4288 (2022)